Focal modulation microscopy
نویسندگان
چکیده
منابع مشابه
Line-scan focal modulation microscopy.
We report the development of a line-scan focal modulation microscope (LSFMM) that is capable of high-speed image acquisition ( > 40 ?? fps ) with uncompromised optical sectioning capability. The improved background rejection and axial resolution of this imaging modality, enabled by focal modulation, are quantified with three-dimensional imaging data obtained from fluorescent beads. The signal-t...
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Focal modulation microscopy (FMM) is an emerging single-photon fluorescence microscopy technique that can provide superior image contrast with sub-micron spatial resolutions at large penetration depths in highly scattering media such as biological tissues, mainly by preserving the signal-to-background ratio (SBR). To achieve this, FMM utilizes the coherence property of the light source, through...
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Focal Modulation Microscopy (FMM) is a single-photon excitation fluorescence microscopy technique which effectively rejects the out-of-focus fluorescence background that arises when imaging deep inside biological tissues. Here, we report on the implementation of FMM in which laser intensity modulation at the focal plane is achieved using acousto-optic modulators (AOM). The modulation speed is g...
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Cells rely on focal adhesions (FAs) to carry out a variety of important tasks, including motion, environmental sensing, and adhesion to the extracellular matrix. Although attaining a fundamental characterization of FAs is a compelling goal, their extensive complexity and small size, which can be below the diffraction limit, have hindered a full understanding. In this study we have used single-m...
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We introduce a technique that improves the sensitivity and resolution and eliminates the nonlocal background of scanned gate microscopy (SGM). In conventional SGM, a voltage bias is applied to the atomic force microscope tip and the sample conductance is measured as the tip is scanned. In the new technique, which we call tip-modulation SGM (tmSGM), the biased tip is oscillated and the induced o...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2008
ISSN: 1094-4087
DOI: 10.1364/oe.16.018764